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RNA Helicases

- Volume 511

Forfatter: info mangler
Bog
  • Format
  • Bog, hardback
  • Engelsk

Beskrivelse

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases.

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Detaljer
  • SprogEngelsk
  • Sidetal584
  • Udgivelsesdato31-07-2012
  • ISBN139780123965462
  • Forlag Academic Press Inc
  • FormatHardback
Størrelse og vægt
  • Vægt1070 g
  • coffee cup img
    10 cm
    book img
    15,2 cm
    22,9 cm

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    ATP DNA Enzyme inhibitors Gene expression. Genomics Interference Hepatitis C virus Catalytic RNA Sumo Regulation Structural analysis Kinetics Proteomics Cyanobacteria RNA Fluorescence Small-angle x-ray scattering Staphylococcus helix Nucleus Motor protein Motor proteins RNA folding Interactions ATPase MRNA Biofilm Förster Resonance Energy Transfer Translocation Equilibrium Thermodynamics Crystal twinning High-throughput screening Mechanism INTRON SAXS Hemolysis RNA Helicase Transcriptional regulation Kinetic analysis Ribosome biogenesis Thermodynamic analysis Nuclear pore complex DEAD-box Helicase Ribozyme Fluorescence polarization Chaperone Cross-linking Conformational dynamics Cryo-EM PTC NMD AAA Unwinding Stopped Flow Rho CRAC 18-O Abiotic gene regulation Base pair Budding yeast (Saccharomyces cerevisiae) Degradosome DExD/H-box Dbp5 eIF4F DEAD-box eIF4A eIF4AI DEAD box RNA helicases iem Hexamer Hexameric helicase Helicase Hippuristanol Isotopic exchange eIF4AII Molecular beacons NAIM NS3 Nucleotide cycle Pateamine A p72 (DDX17) Processing bodies p68 (DDX5) Pre-rRNA Rocaglamides RNA helicases Prp43 Rate Constants Steady-state and transient kinetics RNA decay RIG-I stress granules RNA-binding protein rRNA Processing Hypericin Transcription Termination Translation regulation Inositol Hexakisphosphate Silvestrol Single molecule fluorescence resonance energy transfer mRNA export RECA Quorum sensin Rescue of siRNA downregulation Promoter/reporter activity assays Triparental mating RNA affinity RNA helicase A Translocase SSU processome UPF1 Yeast helicases
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