RNA Abundance Analysis

1. CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana

Veerendra Sharma, Wenguang Zheng, Jun Huang, and David E. Cook



2. Strand-Specific RNA-Seq Applied to Malaria Samples

Xueqing Maggie Lu and Karine Le Roch



3. Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning

Marta Barcala, Carmen Fenoll, and Carolina Escobar



4. Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting

Lena Kr? el, Steffen Ostendorp, Anna Ostendorp, and Julia Kehr



5. Quantitative Analysis of Plant miRNA Primary Transcripts

Jakub Dolata, Andrzej Zielezinski, Agata Stepien, Katarzyna Kruszka, Dawid Bielewicz, Andrzej Pacak, Artur Jarmolowski, Wojciech Karlowski, and Zofia Szweykowska-Kulinska



6. A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq

Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, and Adler Dillman



7. Analysis of RBP Regulation and Co-Regulation of mRNA 3' UTR Regions in a Luciferase Reporter System

Erin L. Sternburg and Fedor V. Karginov



8. Extraction of Small RNAs by Titanium Dioxide Nanofibers

Luis A. Jimenez and Wenwan Zhong



9. Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data

Weixiong Zhang, Xuefeng Zhou, Xiang Zhou, and Jing Xia



10. Purification and Analysis of Chloroplast RNAs in Arabidopsis

Huan Wang and Hailing Jin



11. In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes

Xiaozhen Yao, Hai Huang, and Lin Xu



12. Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs

Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, and Surekha Katiyar-Agarwal



13. Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses

M?lanie Ribeiro Lopes, Pierre Simonet, Gabrielle Duport, Karen Gaget, S?verine Balmand, Akiko Sugio, Jean-Christophe Simon, Nicolas Parisot, and Federica Calevro



14. Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue

Shou-Qiang Ouyang, Gyungsoon Park, Hui-Min Ji, and Katherine A. Borkovich



15. Studying RNA-Protein Interaction Using Riboproteomics

Sonali Chaturvedi and A.L.N. Rao



16. Small RNA Extraction and Quantification