Recombinant DNA Laboratory Manual, Revised Edition

The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. - Comb-bound, three-column, large 9-1/4' x 7-1/2' format- Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts- Necessary reagents and equipment are presented in a checklist at the beginning of each protocol- Techniques for bacteria are complemented with those for Drosophila- Each experiment has been tested in the laboratory by students for five years- Features a complete chapter on computers in the molecular biology laboratory- Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains