Mohamed, A: Protein Chromatography

The separation and isolation of components of a mixture have always captured the fancy of man. Right from the isolation of salt from sea water to the separation of sand and sugar or advanced separation of a complex mixture, several techniques have been invented. Mikhail Semenovich Tswett set the ball rolling with the principle of separation of plant pigments using filter paper and solvent. Today, the technique stands with several branches and applications. There are several types of chromatography on the basis of their principles while the following are the major varieties.Column chromatography, Ion-exchange chromatography, Gel-permeation (molecular sieve) chromatography, Affinity chromatography, Paper chromatography, Thin-layer chromatography, Gas chromatography, Dye-ligand chromatography, Hydrophobic interaction chromatography, Pseudoaffinity chromatography, High-pressure liquid chromatography, Super critical fluid chromatography and Flash chromatography.The techniques have been applied to the separation of compounds, characterization of new molecules, analysis of complex mixtures with both qualitative and quantitative applications. Chromatography offers assistance to all: from high school labs to college experiments to research institutes and the ever-developing biotechnology industry. This book offers collection of the principles, techniques and applications of Protein chromatography with case studies to make the reading more purposeful.