Practical Guide to Protein and Peptide Purification for Microsequencing

Why a Second Edition?The Second Edition provides practical answers to the general question, 'How can I obtain useful sequence information from my protein or peptide?' rather than the more specific question asked in the first edition, 'How can I obtain the N-terminal sequence?' Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides- N-terminal sequencing of proteins with blocked N termini- Internal amino acid sequence analysis after protease digestion in-gel and on-blot- Improved microscale peptide purification methods- Computer analysis of protein sequences- New protocols tested and refined through everyday use in authors' laboratories- Updated reference chapter covering all aspects of protein microsequencing