Plant Gravitropism : Methods and Protocols

1. Plant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds

Sabrina Chin and Elison B. Blancaflor



2. Evaluation of Gravitropism in Non-Seed Plants

Yuzhou Zhang, Lanxin Li, and Jir? Friml



3. Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii

Ashley E. Cannon, Tanya Sabharwal, and Stanley J. Roux



4. A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates

Takehiko Ogura, Christian Goeschl, and Wolfgang Busch



5. Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms

Jinke Chang and Jia Li



6. Using the Automated Botanical Contact Device (ABCD) to Deliver Reproducible, Intermittent Touch Stimulation to Plants

Caleb Fitzgerald, Cullen S. Vens, Nathan Miller, Richard Barker, Matthew Westphall, Johnathan Lombardino, Jerry Miao, Sarah J. Swanson, and Simon Gilroy



7. High-Resolution Kinematic Analysis of Root Gravitropic Bending Using RootPlot

Aditi Bhat, Cody L. DePew, and Gabriele B. Monshausen



8. Staging of Emerged Lateral Roots in Arabidopsis thaliana

Sascha Waidmann and J?rgen Kleine-Vehn



9. Methods for a Quantitative Comparison of Gravitropism and Posture Control Over a Wide Range of Herbaceous and Woody Species

F?lix P. Hartmann, Hugo Chauvet-Thiry, J?r?me Franchel, St?phane Ploquin, Bruno Moulia, Nathalie Leblanc-Fournier, and M?lanie Decourteix



10. The Analysis of Gravitropic Setpoint Angle Control in Plants

Suruchi Roychoudhry, Marta Del Bianco, and Stefan Kepinski



11. A Flat Embedding Method to Orient Gravistimulated Root Samples for Sectioning

Utku Avci and Jin Nakashima



12. Conducting Plant Experiments in Space and on the Moon

Tatsiana Shymanovich and John Z. Kiss



13. Plant Proteomic Data Acquisition and Data Analyses: Lessons from Spaceflight

Proma Basu, Colin P.S. Kruse, Darron R. Luesse, and Sarah E. Wyatt



14. Blueprints for Constructing Microgravity Analogs

Karl H. Hasenstein



15. Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies

Alexander Meyers, Nathan Scinto-Madonich, Sarah Wyatt, and Chris Wolverton



16. Use of Reduced Gravity Simulators for Plant Biological Studies

Ra?l Herranz, Miguel A. Valbuena, Ar?nzazu Manzano, Khaled Y. Kamal, Alicia Villacampa, Malgorzata Ci