PCR Primer Design

SECTION I. Primer design for genotyping



The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species M. Delghandi, M.P. Delghandi, S. Goddard



2. Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.

Amita Pandey, Binu Bhat, Madan L. Aggarwal, Girdhar K. Pandey



3. Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques

Luis Antonio Tortajada-Genaro



4. Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.

Suji George



5. Design of mismatch primers to identify and differentiate closely related (sub)species - application to the authentication of meat products

Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl



6. Primer design for the analysis of closely related species - application of non-coding mtDNA and cpDNA sequences

Lidia Skuza



7. Designing PCR primers for the amplification-refractory mutation system

Majid Komijani, Khashayar Shahin, Esam Ibraheem Azhar, Mohammad Bahram



SECTION II. Primer design for genome-wide identification of specific regions



8. Validation of circular RNAs by PCR

Aniruddha Das, Debojyoti Das, and Amaresh C. Panda



9. Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana

Pinky Dhatterwal, Sandhya Mehrotra, and Rajesh Mehrotra



SECTION III. Primer design for multiplex PCR. Multiplex



10. PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units

Raquel Cuartas, Teresa M. Coque, Fernando de la Cruz, M. Pilar Garcill?n-Barcia



11. Multiplex PCR Design for Scalable Resequencing

Darren Korbie, Matt Trau



SECTION IV. Primer design for qPCR



12. Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR)

Poonam Kanwar, Soma Ghosh, Sibaji K. Sanyal, Girdhar K. Pandey



13. qPrimerDB: A powerful and user-friendly database for qPCR primer design

Wei Chang, Yue Niu, Mengna Yu, Tian Li, Jiana Li, Kun Lu



SECTION V. Primer design for identification of plant and animal viruses



14. PCR primer