Oxidative metabolism and ischaemia in exercise dogs

Knowledge of the possible laboratory alterations caused by exercise is of great importance, especially in dogs that are used in jobs that cause physical strain. The aim of this study was to carry out a laboratory assessment of dogs undergoing exercise tests at different intensities. Heart rate (HR) and rectal temperature (RT) were assessed before exercise, immediately after and 30 minutes after exercise. For the laboratory analysis, complete blood count, total plasma protein, platelet count, serum urea, creatinine, total serum protein, albumin, aspartate aminotransferase activity, creatine kinase and plasma glucose and lactate were assessed at different times, as well as oxidative stress indices using plasma malondialdehyde (MDA) and erythrocyte reduced glutathione (GSH) and ischaemia using ischaemia-modified albumin. Statistical differences were found for HR and RR, as well as erythrocyte count, haemoglobin, haematocrit, platelets, creatinine and glucose levels. The assessment of oxidative stress and the tissue ischaemia marker also varied.