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Several types of fungi were isolated from fast food restaurant which cause food spoilage. The number of colonies isolated from fast food restaurants was higher during October because of increase of temperature and number of shoppers. Some food samples showed high number of Colonies during early morning. The result showed there are some pathogenic fungi present in fast food restaurants like (Aspergillus sp., Alternaria sp., Mucor sp., Rhizopus sp., Saccharomyces sp., Brettanomyces sp.) and the number of colonies on PDA was more than SDA