Gamma Irradiation Effect on WBCs in Blood & Bone Marrow of Balb/c Mice

The phagocytic activity and the respiratory burst of WBCs were assayed using luminol amplified-Chemiluminescence (LA-CL) of whole blood using an ultra-sensitive multi purpose photon counting system. The induced chemiluminescence (CL) signals were acquired, graphed and interpreted to give a clear statistical view about WBCs kinetics and interactions. For irradiation experiments a typical Geiger-Muller (G.M.) tube counter was used and calibrated using standard dose rate meters. For mice irradiation, a heavy shielded radioactive source brought and identified. Using the previously calibrated devices, exposure rates and absorbed dose profile were established. Two BALB/c mice groups were used, a control shielded from radiation, and irradiated was put for the calculated period to accumulate the dose. Mice were dissected for blood and bone marrow collection. Assays were done concerning viability, total and differential blood counting, CL assays were done firstly on whole blood to verify WBCs total reactive activity, reaction time, peak activity and secondly on bone marrow to evaluate the total reaction time, peak activity values, and the reactive activity per micro-liter unit.