Dengue Virus : Methods and Protocols

Part I: Virus and Virus Protein Production



1. Virus Propagation and Plaque Assay for Dengue Virus

Pedro Henrique Carneiro, Lucas Mendes-Monteiro, and Ronaldo Mohana-Borges



2. Transmission Electron Microscopy Studies of Dengue Viruses

Gabriela Cardoso Caldas, Ortrud Monika Barth, and Debora Ferreira Barreto-Vieira



3. Production of Properly Folded NS1 Protein in Bacterial Cells

Diego Allonso



4. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System

Diego R. Coelho and Pedro Henrique Carneiro



5. Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals

Aicha Gharbi-Ayachi, Abbas El Sahili, and Julien Lescar



6. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly

Szu-Chia Hsieh, Wen-Yang Tsai, and Wei-Kung Wang



7. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons

Thu M. Cao and Richard J. Kuhn



Part II: Interaction between Dengue Virus and Cellular Proteins



8. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis

Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, and Laura C. Estrada



9. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions

Jonas Nascimento Conde



10. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry

Vivian Huerta and Yassel Ramos



Part III: Immunopathogensis and Diagnosis



11. Molecular Diagnosis Of Dengue

Priscila C.G. Nunes, Monique R.Q. Lima, and Fl?via B. dos Santos



12. Serological Diagnosis of Dengue

Monique R.Q. Lima, Priscila C.G. Nunes, and Fl?via B. dos Santos



13. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies

Jonas Nascimento Conde, Megan Mladinich, William Schutt, and Erich R. Mackow



14. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus

Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, and Luciana Barros de Arruda



15. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells

Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, and Guilherme Ferreira Silveira