Basophils and Mast Cells : Methods and Protocols

Part I: Introduction and Historical Background

1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease

Ana Olivera and Juan Rivera

 

Part II: Obtaining the Cells: Purification, Culture, and Counting

2. Purification of Basophils from Peripheral Human Blood

Franco H. Falcone and Bernhard F. Gibbs

 

3. Mast Cell Purification Protocols

Jasmine Farrington, Elizabeth P Seward, and Peter T Peachell

 

4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells

Hans Jürgen Hoffmann

 

5. Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs)

Yanyan Luo, Stefan Frischbutter, Jörg Scheffel, and Frank Siebenhaar

 

6. Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34+ Progenitor Cells

Donald MacGlashan Jr.

 

7. Generation of Mast Cells from Murine Stem Cell Progenitors

Emily J. Swindle

 

8. Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model

Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc

 

9. The Absolute Basophil Count

Elena Borzova

 

Part III: Basophil/Mast Cell Lines

10. Mast Cell and Basophils Cell Lines: A Compendium

Egle Passante

 

11. Use of Humanized RBL Reporter Systems for Detection of Allergen-specific IgE Sensitization in Human Serum

Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone

 

12. Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for Detection of Allergen-specific IgE in Patient Sera Using Allergen Microarrays

Marina Kalli, Marcos J. C. Alcocer, Andrew J. Blok, and Franco H. Falcone

 

13. NPY-mRFP Rat Basophil Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation

Nafal Barwary, Daniel Wan, and Franco H. Falcone

 

14. Use of Engineered Nanoparticles (ENPs) for the Study of High-affinity IgE Receptor FceRI Receptor Engagement and Rat Basophil Leukemia (RBL) Cell degranulation

Alelwani W, Alharbi RA , Wan D , Vllasaliu D, Falcone FH , and Stolnik S

  Part IV: Diagnostic Applications 15. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo

 

16. Basophil Activation Experiments in Immediate Drug Hypersensitivity: More Than a Diagnostic Aid

Didier G Ebo, Jessy Elst, Athina Van Gasse, Leander De Puysseleyr, Margaretha Faber, Margo M Hagendorens, Lina Mayorga, Christel Mertens , Chris H Bridts, Luc S De Clerck, and Vito Sabato

 

17. Basophil Activation Techniques: Staining of Exteriorized Granule Matrix Jessy Elst, Vito Sabato, Margo M Hagendorens, Michel van Houdt, Margaretha A. Faber, Chris H Bridts, Didier G Ebo, and Christel Mertens  

18. Measurement and Functional Analysis of the Mas-related G protein-coupled receptor MRGPRX2 on Human Mast Cells and Basophils Jessy Elst, Vito Sabato, Margo M Hagendorens, Michel van Houdt, Margaretha A. Faber, Chris H Bridts, Didier G Ebo, and Christel Mertens

 

19. The Mast Cell Activation Test (MAT)

Rajia Bahri and Silvia Bulfone-Paus

 

Part V: Whole Cells: Functional Studies

20. Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique

Victor G. Bechmann, Anett