Bacterial Vaccines : Methods and Protocols

Preface...

Table of Contents...

Contributing Authors... 1 Vaccine Design by Reverse Vaccinology and Machine Learning

Edison Ong and Yongqun He 2 Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates against Shigella flexneri

Chiuan Yee Leow, Candy Chuah, Abdul Majeed Abu Bakar, Mohd Nor Norazmi, and Chiuan Herng Leow 3 Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation

Mark Reglinski 4 Rapid Surface Shaving for Proteomic Identification of Novel Surface Antigens for Vaccine Development

Laurence Don Wai Luu and Ruiting Lan 5 Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria

Milan Obradovic and Heather L. Wilson 6 Panproteome Analysis of the Human Antibody Response to Bacterial Vaccines and Challenge

Joseph J. Campo and Amit Oberai 7 Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines

Jasmin Fertey, Bastian Standfest, Jana Beckmann, Martin Thoma, Thomas Grunwald, and Sebastian Ulbert 8 Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria

Somshukla Chaudhuri, Nikolas F. Ewasechko, Luisa Samaniego-Barron, Jamie E. Fegan, and Anthony B. Schryvers 9 Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit Vaccine Delivery

Lili Zhao, Sahra Bashiri, Istvan Toth, and Mariusz Skwarczynski 10 Multiepitope Fusion Antigen - MEFA, An Epitope- and Structure-Based Vaccinology Platform for Multivalent Vaccine Development

Siqi Li, Kuo Hao Lee, and Weiping Zhang 11 Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development

R?gis Stentz, Ariadna Miquel-Clop?s, and Simon R. Carding 12 Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures

Jun Kawamoto and Tatsuo Kurihara 13 Glycine Induction Method: Effective Production of Immunoactive Bacterial Membrane Vesicles with Low Endotoxin Content

Satoru Hirayama and Ryoma Nakao 14 Methods for Assessment of OMV/GMMA Quality and Stability

Francesca Micoli, Renzo Alfini, and Carlo Giannelli 15 Production of Vaccines using Biological Conjugation

Emily J. Kay and Vanessa S. Terra 16 Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines against Bacterial Pathogens

Anneliese S. Ashhurst, Cameron C. Hanna, Richard J. Payne, and Warwick J. Britton 17 Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy

Thomas Rice and Beth Holder 18 Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma

Frances Alexander, Emily Brunt, Holly Humphries, Breeze Cavell, Stephanie Leung, Lauren Allen, Rachel Halkerston, Elodie Lesne, Elizabeth Penn, Stephen Thomas, Andrew Gorringe, and Stephen Taylor 19 Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets

Evgeny A. Semchenko, Freda E.C. Jen, Michael P. Jennings, and Kate L. Seib 20 Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum

Helen Wagstaffe, Scott Jones, Marina Johnson, and David Goldblatt 21 Neisseria lactamica Controlled Human Infection Model

Adam P. Dale, Diane F. Gbesemete, Robert C. Read, and Jay R. Laver 22 Analyzing Macrophage Infection at the Organ Level

Ryan G. Hames, Zydrune Jasiunaite, Joseph J. Wanford, David Carreno, Wen Y. Chung, Ashley R. Dennison, an