Atomic, Molecular, and Optical Physics: Atoms and Molecules

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradation Labeling a protein with fluorophores using NHS ester derivitization Immunoaffinity purification of proteins Proteolytic affinity tag cleavage Purification of GST-tagged proteinsCombined with the other two volumes, this text is a comprehensive treatment of the key experimental methods of atomic, molecular, and optical physics, as well as an excellent experimental handbook for the field. Thewide availability of tunable lasers in the past several years has revolutionized the field and lead to the introduction of many new experimental methods that are covered in these volumes. Traditional methods are also included to ensure that the volumes will be a complete reference source for the field.